Detection of 16SrRNA gene Polymorphism using PCR for some bacterial species of clinical samples

Fifty samples were collected from patients suffering the presence of infections in skin, including wounds and burns, inside Fallujah Teaching Hospital. The period September to December 2021, after isolation, transplantation, microscopic molecular diagnosis, 30 obtained on which bacteria appeared Including (E. coli K. pneumoniae), constitute about 60 % total samples. test showed its development different culture media. These isolates cultured Chrom agar, MacConkey Blood agar. It was found that there a variation species, where highest percentage pneumoniae) appeared, biochemical tests also used. By using diagnosis 16srRNA gene detect between by PCR technique. results sequence analysis examined blast National Center for Biotechnology Information (NCBI) BioEdit program polymorphism.